Development of a Human Intestinal Organoid Model for <i>In Vitro</i> Studies on Gut Inflammation and Fibrosis

<div>Epithelial responses to proinflammatory cytokines decrease during passaging. IL-1<i>α</i> and TNF-<i>α</i> (2C) induced the mRNA expression of OCLN in passage 4 (a), CLDN1 in passages 2 and 8 (b), ZO1 in passage 4 (c), and JAMA in passages 2, 8, and 10 (d). Concentrations of cytokines used: IL-1<i>α</i> 5 ng/ml, TNF-<i>α</i> 50 ng/ml. All experiments were performed in triplicate. The gene expression of each studied gene was normalized against GAPDH gene expression in the same sample using the 2<sup>-<i>ΔΔ</i>Ct</sup> method. In every passage, expression levels of treated organoids were normalized against those of the control ones. 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Stem Cells International

fig7

Figure 7

Figure 7: Development of a Human Intestinal Organoid Model for <i>In Vitro</i> Studies on Gut Inflammation and Fibrosis 

Stem Cells International

Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis

Figure 7