Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis

Table 1

Gene-specific primers used in real-time PCR.

Gene	Forward	Reverse	Reference
GAPDH	GACATCAAGAAGGTGGTGAA	TGTCATACCAGGAAATGAGC	[9]
Collagen type I	CCCTGGAAAGAATGGAGATGAT	ACTGAAACCTCTGTGTCCCTTCA
Collagen type III	GCTCTGCTTCATCCCACTATTA	TGCGAGTCCTCCTACTGCTAC
Fibronectin	CCAGTCCACAGCTATTCCTG	ACAACCACGGATGAGCTG
α-SMA	AATGCAGAAGGAGATCACGG	TCCTGTTTGCTGATCCACATC
TF	TTCAGTGTTCAAGCAGTGATTCC	ATGATGACCACAAATACCACAGC
CD90	CGCTCTCCTGCTAACAGTCTT	CAGGCTGAACTCGTACTGGA	[41]
CCL2	AGGAAGATCTCAGTGCAGAGG	AGTCTTCGGAGTTTGGGTTTG	[42]
CCL20	GCTGCTTTGATGTCAGTGC	GCAGTCAAAGTTGCTTGCTTC	[43]
CXCL1	GCCCAAACCGAAGTCATAGCC	ATCCGCCAGCCTCTATCACA	[44]
CXCL8	TGGGTGCAGAGGGTTGTG	CAGACTAGGGTTGCCAGATTTA	[42]
CXCL10	CCTGCTTCAAATATTTCCCT	CCTTCCTGTATGTGTTTGGA
CXCL11	GACGCTGTCTTTGCATAGGC	GGATTTAGGCATCGTTGTCCTTT	[45]
CLDN1	CGATGCTTTCTGTGGCTAA	AGTGGCTGACTTTCCTTGT	[46]
OCLN	CCTATAAATCCACGCCGGTTC	TCAAAGTTACCACCGCTGCTG
ZO1	AACAGCCCTACCCATCTCG	CGTGGAAAGTACCCTCGTT
JAMA	CGAGAGGAAACTGTTGTGCC	AACGAGTCTGGTGGTGTCTC	[47]