Heme Oxygenase-1-Modified Bone Marrow Mesenchymal Stem Cells Combined with Normothermic Machine Perfusion Repairs Bile Duct Injury in a Rat Model of DCD Liver Transplantation via Activation of Peribiliary Glands through the Wnt Pathway

Figure 1

Process of DCD liver transplantation and engraftment of bone marrow mesenchymal stem cells in implanted livers. (a) Normal liver with a pink, homogeneous appearance. (b) After 30 minutes of warm ischemia, the liver was congested with heterogeneous dark purple areas. (c) After NMP for 4 h, the liver was yellowish-brown without congestion. (d) The blood flow of the liver was restored after implantation, and the appearance of liver basically returned to normal. In vivo (e) and in vitro (f) imaging showing engraftment of BMMSCs in liver. Frozen sections showing that the survival rate of HO-1/BMMSCs (g) in the liver was higher than that of BMMSCs (h). Western blotting (i) and PCR (j) showing high HO-1 expression in the HBP group at 7 days after liver transplantation (). , , and . DCD: donors after circulatory death; NMP: normothermic machine perfusion; BMMSCs: bone marrow mesenchymal stem cells; HO-1: heme oxygenase-1; HBP: HO-1/BMMSCs plus NMP.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)