Palmitic Acid Methyl Ester Enhances Adipogenic Differentiation in Rat Adipose Tissue-Derived Mesenchymal Stem Cells through a G Protein-Coupled Receptor-Mediated Pathway

Figure 1

PAME increases adipogenic differentiation in rAD-MSCs. Treatment with PAME (10-100 μM) in adipogenic induction medium for 12 days enhanced rAD-MSC adipogenic differentiation evidenced by Oil Red O staining (a) and quantitation of lipid droplets (b). Values shown in parentheses represent the number in each group. PAME (50 μM) significantly increased the mRNA levels of Pparγ (c) () and Gpd1 (d) () and the protein level of PPARγ (e) (). (f) The effect of PAME on adipogenic differentiation was blocked by 0.5% BSA (). (g) Veh (0.1% methanol), SAME (50 μM), and PA (50 μM) did not enhance adipogenic differentiation of rAD-MSCs (). All data represent . , versus the CM group; ^#, versus the AIM group; ^$, versus the AIM+PAME group. CM: culture medium; AIM: adipogenic induction medium; BSA: bovine serum albumin; Veh: vehicle.

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