Adipose-Derived Stem Cell-Derived Extracellular Vesicles Inhibit the Fibrosis of Fibrotic Buccal Mucosal Fibroblasts via the MicroRNA-375/FOXF1 Axis

<div>ADSC-EVs are successfully extracted. (a) ADSCs were transfected with miR-375 mimic or NC mimic, and the EVs (EVs-miR-375/EVs-NC) and EVs from ADSCs were extracted; the transfection efficiency was detected using RT-qPCR. (b) ADSC-EVs were separated and purified using ultracentrifugation methods, and their morphology and size of ADSC-EVs were analyzed under the TEM. (c) The particle size of EVs was measured using NanoSight. (d) The expression of CD9, CD63, and calnexin was detected using WB. GW4869: treatment of ADSC conditioned medium supplemented with EV inhibitor GW4869; EVs: treatment of ADSC-EVs. (e) miR-375 expression in EVs was detected using RT-qPCR. 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Stem Cells International

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Figure 2

Figure 2: Adipose-Derived Stem Cell-Derived Extracellular Vesicles Inhibit the Fibrosis of Fibrotic Buccal Mucosal Fibroblasts via the MicroRNA-375/FOXF1 Axis 

Figure 2 | Adipose-Derived Stem Cell-Derived Extracellular Vesicles Inhibit the Fibrosis of Fibrotic Buccal Mucosal Fibroblasts via the MicroRNA-375/FOXF1 Axis 