mTORC2 Activation Mediated by Mesenchymal Stem Cell-Secreted Hepatocyte Growth Factors for the Recovery of Lipopolysaccharide-Induced Vascular Endothelial Barrier

<div>Effects of mTORC1 and mTORC2 to HGF on VE-cadherin expression in LPS-induced PMVEC barrier. Lentivirus vector-mediated raptor and rictor knockdown in PMVECs (shRaptor and shRictor as knockdown, shRNA-control as negative control) was conducted. PMVECs were treated with HGF (20 ng/ml), with or without stimulation with LPS (100 ng/ml) for 4 h. (a) Flow cytometry scatter plot of 4 h VE-cadherin expression with HGF in LPS-induced PMVEC-shRaptor. (b) Flow cytometry cell counts (%) of 4 h VE-cadherin expression with HGF in LPS-induced PMVEC-shRaptor. (c) Flow cytometry scatter plot of 4 h VE-cadherin expression with HGF in LPS-induced PMVEC-shRictor. (d) Flow cytometry cell counts (%) of 4 h VE-cadherin expression with HGF in LPS-induced PMVEC-shRictor. 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Stem Cells International

fig3

Figure 3

Figure 3: mTORC2 Activation Mediated by Mesenchymal Stem Cell-Secreted Hepatocyte Growth Factors for the Recovery of Lipopolysaccharide-Induced Vascular Endothelial Barrier 

Figure 3 | mTORC2 Activation Mediated by Mesenchymal Stem Cell-Secreted Hepatocyte Growth Factors for the Recovery of Lipopolysaccharide-Induced Vascular Endothelial Barrier 