Gastric Cancer Mesenchymal Stem Cells Inhibit NK Cell Function through mTOR Signalling to Promote Tumour Growth

Figure 2

GCMSCs directly attenuated the degranulation ability of NK cells. (a) FCM gating strategy for enriched NK cells (CD56⁺) from the peripheral blood of healthy donors. (b) Representative histograms of CD107a in freshly isolated NK cells. (c) NK cells cocultured with or without GCMSCs at a 1 : 1 ratio for 48 h were analysed through flow cytometry for CD107a expression. (d) Quantification of CD107a expression in NK cells shown in (c). Data are presented as . Paired test, . (e) Analysis of CD107a+ NK cells. Production of CD107a in response to GCMSCs followed by stimulation with MKN-45 for 5 h was measured. (f) Enriched NK cells cocultured with MKN-45 cells with or without GCMSC for 7 days. Representative histograms of CD107a and PD-1 are shown. (g) NK92 cells cocultured with GCMSCs for 48 h. Representative histograms of CD107a are shown. (h) Quantification of CD107a expression. Data are presented as . Paired test, . (i) NK92 cells cocultured with HGC-27 and/or GCMSCs. Representative histograms of CD107a in NK92 cells are shown.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)