Gastric Cancer Mesenchymal Stem Cells Inhibit NK Cell Function through mTOR Signalling to Promote Tumour Growth

<div>GCMSC-CM effectively induced an exhaustion state in NK cells. (a) NK92 cells treated with GCMSC-CM for 48 h were analysed through flow cytometry for CD107a. (b) Quantification of the percentage of positive cells shown in (a). (c) Analysis of CD107a expression in NK cells isolated from healthy donors cultured with or without GCMSC-CM through flow cytometry. (d) Quantification of the effect of GCMSC-CM on NK cell proliferation by CCK8. (e, f) Representative FCM plot of the indicated molecules in NK92 cells treated with GCMSC-CM. Results are representative of three independent experiments. (g) Quantification of the percentage and mean fluorescence intensity (MFI) of the indicated molecules shown in (e, f). 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Stem Cells International

fig3

Figure 3

Figure 3: Gastric Cancer Mesenchymal Stem Cells Inhibit NK Cell Function through mTOR Signalling to Promote Tumour Growth 