Gastric Cancer Mesenchymal Stem Cells Inhibit NK Cell Function through mTOR Signalling to Promote Tumour Growth

Figure 6

Attenuated effector function in NK cells is partly mediated by mTOR signalling. (a) Western blot analysis of mTOR downstream activation (pS6) in NK92 cells cultured in GCMSC-CM for 48 h. (b) pS6 expression in NK92 cells was detected after treatment with or without rapamycin (100 ng/ml) for 48 h by using a Western blot. (c, d) NK92 cells were incubated with rapamycin and GCMSC-CM. Representative histograms of indicated molecules of CD107a and perforin are shown. (e) Indirect Transwell cocultures between GCMSCs and NK92 and TGF-β expression in GCMSC were evaluated using Western blot. GCMSCs derived from three different patients.

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