Effects of Changes in Osmolarity on the Biological Activity of Human Normal Nucleus Pulposus Mesenchymal Stem Cells

Figure 3

(a) Alizarin Red staining showed that cells formed mineralized nodules. (b) Oil Red O staining revealed that cells produced intracellular lipid vacuoles. (c) Alcian Blue staining indicated that cells exhibited sulfated proteoglycan.

(a)

(b)

(c)