miR-100-5p Promotes Epidermal Stem Cell Proliferation through Targeting MTMR3 to Activate PIP3/AKT and ERK Signaling Pathways

Figure 3

MTMR3 is a target of miR-100-5p. (a) Sequence alignment of miR-100-5p with the 3UTR of human MTMR3 mRNA. (b) Luciferase activities in HEK293T cells cotransfected with luciferase reporter constructs containing the WT or mutated 3UTR fragment of MTMR3 mRNA (MTMR3-WT, MTMR3-Mut) and miR-100-5p mimics (miR-100-5p) or negative control miRNA (NC) for 24 h. (c, d) Human EpSCs were transfected with NC or miR-100-5p. MTMR3 mRNA and protein levels were examined after 24 and 48 h, respectively. Data are , . and , compared with cells transfected with NC.

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