miR-100-5p Promotes Epidermal Stem Cell Proliferation through Targeting MTMR3 to Activate PIP3/AKT and ERK Signaling Pathways

<div>MTMR3 negatively regulates epidermal stem cell proliferation. (a) Human epidermal stem cells (EpSCs) transfected with MTMR3 siRNA or control siRNA for 24 h were examined for the MTMR3 mRNA level by RT-qPCR or cultured for different periods of time to determine cell proliferation by MTT assay. (b) EpSCs transfected with MTMR3 siRNA or control siRNA for 48 h were examined for cell cycle distribution by flow cytometry. 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313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,46.402,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,49.366,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,55.606,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,61.846,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>,</span> compared with cells transfected with control siRNA for the same period of time.</div>

Stem Cells International

fig4

Figure 4

Figure 4: miR-100-5p Promotes Epidermal Stem Cell Proliferation through Targeting MTMR3 to Activate PIP3/AKT and ERK Signaling Pathways 