miR-100-5p Promotes Epidermal Stem Cell Proliferation through Targeting MTMR3 to Activate PIP3/AKT and ERK Signaling Pathways

Figure 6

miR-100-5p upregulates cyclins expression through MTMR3. (a, b, and d) Human epidermal stem cells (EpSCs) transfected with miR-100-5p mimics (miR-100-5p) or negative control miRNA (NC); MTMR3 siRNA or control siRNA for 24 h was examined for the expression of MTMR3 and cyclins D1, E1, and A2 at mRNA and protein levels. (c, e) EpSCs cotransfected with NC and control vector (Vector), miR-100-5p and Vector, or miR-100-5p and MTMR3 expression plasmid (MTMR3) for 24 h were examined for the expression of MTMR3 and cyclins D1, E1, and A2 at mRNA (c) and protein levels (e). Data are shown as , . , , and , compared with cells transfected with (a, d) NC, (b) control siRNA, or (c, e) NC and Vector; , , and , compared with cells transfected with (c, e) miR-100-5p and Vector or (d) control siRNA.

(a)

(b)

(c)

(d)

(e)