miR-100-5p Promotes Epidermal Stem Cell Proliferation through Targeting MTMR3 to Activate PIP3/AKT and ERK Signaling Pathways

Figure 8

miR-100-5p promotes epidermal stem cell proliferation through MTMR3-mediated activation of ERK. (a) Human epidermal stem cells (EpSCs) transfected with miR-100-5p mimics (miR-100-5p) or negative control miRNA (NC); MTMR3 siRNA or control siRNA for 24 h was examined for ERK phosphorylation. (b) EpSCs cotransfected with NC and control vector (Vector), miR-100-5p and Vector, or miR-100-5p and MTMR3 expression plasmid (MTMR3) for 24 h were examined for ERK phosphorylation. (c) EpSCs transfected with NC or miR-100-5p for 24 h were treated with 10 μM PD98059 or control solvent (DMSO) for 2 or 3 days; cell proliferation was measured by MTT assay. Data are shown as , . , , and , compared with cells transfected with (a) NC, (b) NC + Vector, or (c) cells transfected with NC and treated with DMSO; and , compared with cells transfected with (a) control siRNA, (b) miR-100-5p + Vector, or (c) cells transfected with miR-100-5p and treated with DMSO.

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