miR-100-5p Promotes Epidermal Stem Cell Proliferation through Targeting MTMR3 to Activate PIP3/AKT and ERK Signaling Pathways

Figure 9

miR-100-5p promotes EpSC proliferation in the organotypic skin through MTMR3. (a) Human EpSCs were cultured with 20 μg ADSC-Exos (Exos), miR-100-5p-enriched ADSC-Exos (miR-100-Exos), or same volume of PBS for different periods of time and examined for cell proliferation by MTT assay. (b–d) Human skin explants intradermally injected with PBS, Exos, miR-100-Exos, or miR-100-Exos + MTMR3 expression plasmid (MTMR3) were cultured for 2 and 5 days. Histology was examined by HE staining; cell proliferation and EpSCs were examined by immunohistochemical staining of PCNA, β1 integrin and α6 integrin. miR-100-5p and MTMR3 mRNA levels were examined by RT-qPCR. Data are presented as the , . , , and , comparison between Exo- and PBS-treated EpSCs or skin explants; , , and , comparison between miR-100-Exo- and PBS-treated EpSCs or miR-100-Exo- and Exo-treated skin explants; , , and , comparison between miR-100-Exo- and Exo-treated EpSCs or miR-100-Exos + MTMR3- and miR-100-Exos-treated skin explants. Images in (b) are representative results of 3 independent experiments. Scale .

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