Identification and Validation of a Potential Stemness-Associated Biomarker in Hepatocellular Carcinoma

Figure 3

Protein-protein network (PPI) construction and the relationship between hub gene BUB1, clinical, and stemness characteristics in HCC samples from the TCGA cohort. (a) The PPI construction of candidate genes in the blue module. (b) The hub gene was identified using the Cytoscape software. (c) The levels of BUB1 in various tumor and normal tissues were examined using TIMER2.0. (d) Correlation between BUB1 expression and mRNAsi group. (e–k) Differences in BUB1 expression and clinical characteristics: grade (e), stage (f), fetoprotein value (g), vital status (h), cancer status (i), or vascular invasion (j). (k) The correlations between BUB1 expression and upregulated genes in human embryonic stem cell lines from MSigDB. (l) The correlations between BUB1 expression and specific stemness markers. (m, n) Heatmap of the significantly differential hallmarks and pathways of BUB1 subtypes in HCC.

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