Identification and Validation of a Potential Stemness-Associated Biomarker in Hepatocellular Carcinoma

Figure 5

Validation of stemness characteristics and expression of BUB1 in HCC patients from the GEO and ICGC cohorts and TRCs. (a, b) The correlations between BUB1 expression and upregulated genes in human embryonic stem cell lines in the ICGC (a) and GSE14520 (b) cohorts. (c–f) GSEA of BUB1 in the ICGC (c, e) and GSE14520 (d, f) cohorts. (g) qRT-PCR of BUB1 expression in Hep3B and Huh7 vs. LO2. (h) Representative images of liver TRC spheroids. Scale bars, 20 μm. (i, j) qRT-PCR of BUB1 expression in Hep3B and Huh7 liver TRCs vs. control cells. (k, l) qRT-PCR of BUB1 expression in HT29 and HCT116 colon TRCs vs. control cells. Data are shown as the (); , , , and .

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