Hypoxia Enhances HIF1α Transcription Activity by Upregulating KDM4A and Mediating H3K9me3, Thus Inducing Ferroptosis Resistance in Cervical Cancer Cells

Figure 1

Hypoxia inhibited CC cell viability and induced ferroptosis resistance. SiHa and Hela cell lines were treated with hypoxia for 0.5, 1, 2, 4, and 12 h, or SiHa and Hela cell lines cultured in normoxic and hypoxic conditions for 2 h were co-incubated with ferroptosis inducer Erastin for 24 h. (A) Cell viability was determined by MTT assay; (B-D) The intracellular iron concentration, MDA, and GSH levels were measured using colorimetry method. The experiments were conducted in 3 replicates independently. The data were expressed as mean ± standard deviation. One-way ANOVA was adopted for comparisons among groups with Tukey’s multiple comparison test as the post hoc test; vs. the normal group, p <0.05; # vs. the Hypo-2 h group, p <0.05; & vs. the Normal + Erastin group, p <0.05.

(a)

(b)

(c)

(d)