Hypoxia Enhances HIF1α Transcription Activity by Upregulating KDM4A and Mediating H3K9me3, Thus Inducing Ferroptosis Resistance in Cervical Cancer Cells

Figure 4

KDM4A mediated H3K9me3 to enhance the transcriptional activity of HIF1α in hypoxia. SiHa and Hela cell lines were interfered with using shKDM4A and treated with hypoxia for 2 h. (A) The analysis map of HIF1α expression profile in TCGA CC database was obtained from the UALCAN website; (B) ENCORI pan cancer coexpression analysis showed a positive correlation between KDM4A mRNA level and HIF1α mRNA level in CC cells; (C) The mRNA levels of KDM4A and HIF1α in CC cells were detected by RT-qPCR; (D) The protein levels of KDM4A, HIF1α and H3K9me3 in CC cells were detected by Western blot; (E) The level of H3K9me3 in HIF1A promoter was detected by ChIP assay. The experiments were conducted in 3 replicates independently. The data were expressed as mean ± standard deviation. The independent t test was used for comparison among groups. , #compared with the IgG group, P <0.05. CESC: Cervical squamous cell carcinoma.

(a)

(b)

(c)

(d)

(e)