Hypoxia Enhances HIF1α Transcription Activity by Upregulating KDM4A and Mediating H3K9me3, Thus Inducing Ferroptosis Resistance in Cervical Cancer Cells

Figure 5

Hypoxia upregulated KDM4A/HIF1α resistance to ferroptosis in CC cells. SiHa and Hela cell lines were simultaneously transfected with shKDM4A and pcDNA3.1-HIF1α, followed by hypoxia treatment. (A-B) The mRNA and protein levels of HIF1α were detected by RT-qPCR and Western blot; (C) The level of H3K9me3 in HIF1A promoter was detected by ChIP assay; (D-E) The transfection efficiency of pcDNA3.1-HIF1α was detected by RT-qPCR and Western blot; (F) Cell viability was determined by MTT assay; (G-I) The intracellular iron concentration, MDA and GSH levels were measured using colorimetry method. The experiments were conducted in 3 replicates independently. The data were expressed as mean ± standard deviation. The independent t test was employed for comparisons between two groups. .

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