Hypoxia Enhances HIF1α Transcription Activity by Upregulating KDM4A and Mediating H3K9me3, Thus Inducing Ferroptosis Resistance in Cervical Cancer Cells

Figure 6

Hypoxia activated the HIF1/HRE pathway and upregulated expression of iron transporter in CC cells. SiHa and Hela cell lines were treated with hypoxia for 2 h. (A-B) Cell iron uptake and release were determined using isotope ⁵⁵Fe tracing method; (C-D) The mRNA levels of TfR1 and DMT1 in CC cells were detected by RT-qPCR; (E-F) The protein levels of TfR1 and DMT1 in CC cells were detected by Western blot; (G) The CC cells were transfected with different luciferase plasmids to detect luciferase activity. The experiments were conducted in 3 replicates independently. The data were expressed as mean ± standard deviation. The non-paired t test was utilized for comparisons among groups. .

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