Conjugated Linoleic Acid Treatment Attenuates Cancerous features in Hepatocellular Carcinoma Cells

Figure 5

In silico data analysis. (a) Analysis of protein-protein interaction (PPI) between HNF4a and classical EMT-related proteins highlighting a strong association between HNF4a and CDH1, SNAI1, SNAI2, and ZEB2. (b) Correlation coefficient analysis between expression of HNF4a and top 50 epithelial and mesenchymal genes derived from (PMID: 25214461) across liver carcinoma samples (). These results indicate a strong negative association () between the upregulation of HNF4a expression and the reduction of mesenchymal-related genes involved in EMT program. Compared to the mesenchymal genes, a weak negative association () was observed between HNF4a expression and epithelial genes. These results highlighted that induction of HNF4a might suppress the mesenchymal phenotype and deteriorate the metastatic capacity of liver carcinoma cells. (c) The scatter plot depicts positive association between HNF4a and the expression levels of ZO1 and ALB across 372 liver carcinoma samples from TCGA.

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