Research Article
Extracellular Vesicles Derived from Adipose Mesenchymal Stem Cells Promote Peritoneal Healing by Activating MAPK-ERK1/2 and PI3K-Akt to Alleviate Postoperative Abdominal Adhesion
Figure 4
Impact of ADSC-EVs on peritoneal inflammation and apoptosis. (a) Hematoxylin-eosin staining of injured tissues (40x). Black tetragonum represents the accumulation of plasma cells, granulocytes, and macrophages (200x). (b–f) The expression of CD163+, CCR7+, IL-10+, IL-6+, and COX-2+ was evaluated at 4 days postsurgery by immunofluorescence assay. (g) Impact of ADSC-EVs on peritoneal apoptosis. Cellular expression of caspase 3+ at 4 days after surgery evaluated by immunofluorescence assay. (h) Positive cell ratio of inflammation-related factors and caspase 3+ cells at 4 days (). Bars, 50 μm. Data are represented as mean ± SD. , vs. control; .