Extracellular Vesicles Derived from Adipose Mesenchymal Stem Cells Promote Peritoneal Healing by Activating MAPK-ERK1/2 and PI3K-Akt to Alleviate Postoperative Abdominal Adhesion

Figure 8

ADSC-EVs promote the proliferation and migration of cells via the PI3K/AKT and MAPK/ERK1/2 signaling. (a–c) Western-blot analysis of protein levels of p-Akt and p-ERK1/2 induced by different concentrations of ADSC-EVs. (d–g) LY294002 and PD98059 inhibit the activation of PI3K-AKT and MAPK-ERK1/2 induced by ADSC-EVs, respectively. (h, i) EdU assay showed that ADSC-EV-mediated RPMC proliferation was suppressed by inhibitors LY294002 and PD98059. (j, k) Transwell and (l, m) scratch assays showed that ADSC-EVs enhanced RPMC migration at 24 h, but this effect was significantly reduced by inhibitors LY294002 and PD98059. Bars, 100 μm; data are represented as the . , vs. control group; . . ^#, vs. 25 μg/mL group; ^## and ^##. ^&, vs. 50 μg/mL group; ^&&; ^&&&&.

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