Mesenchymal Stem Cells Alleviate Renal Fibrosis and Inhibit Autophagy via Exosome Transfer of miRNA-122a

Figure 1

MSC-derived exosomes relieve fibrosis caused by TGFβ in HK-2 cells via regulation of mTOR signaling and downstream autophagy. (a) TGFBR1, α-SMA, Col1a1, fibronectin, and E-cadherin mRNA levels were evaluated by qPCR in HK-2 cells. (b) Immunoblotting analysis for TGFBR1, α-SMA, Col1a1, fibronectin, and E-cadherin expression in HK-2 cells. Quantitative analysis of TGFBR1, α-SMA, Col1a1, fibronectin, and E-cadherin expression is shown on the right ( independent experiments). (c) LC3 II/I, p62, Beclin 1, ATG7, mTOR, p-mTOR, AKT, and p-AKT protein expression was evaluated by western blotting in HK-2 cells, and the quantitative analysis is shown on the right ( independent experiments). Results are expressed as the . , , and .

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