Cebpb Regulates Skeletal Stem Cell Osteogenic Differentiation and Fracture Healing via the WNT/β-Catenin Pathway

Figure 3

Cebpb knockdown promoted mSSC osteogenic differentiation. (a) Expression of osteogenic differentiation markers determined by western blot in the Cebpb inhibition group and the control group. (b) Quantification of result (a). (c) Expression of osteogenic differentiation markers determined by qPCR in the Cebpb inhibition group and the control group. (d) Alizarin red S staining and alkaline phosphatase staining of mSSCs in the Cebpb inhibition group and the control group. Scale bar: 200 μm. (e) Quantification of alkaline phosphatase staining of result (d). (f) Quantification of alizarin red S staining of result (d). (g) Expression of osteogenic differentiation markers determined by western blot in the Cebpb overexpression group and the control group. (h) Quantification of result (g). (i) Expression of osteogenic differentiation markers determined by qPCR in the Cebpb overexpression group and the control group. (j) Alizarin red S staining and alkaline phosphatase staining of mSSCs in the Cebpb overexpression group and the control group. Scale bar: 200 μm. (k) Quantification of alkaline phosphatase staining of result (j). (l) Quantification of alizarin red S staining of result (j). Cebpb-sh1: mSSCs stably expressing the Cebpb-shRNA1 sequence; Cebpb-sh2: mSSCs stably expressing the Cebpb-shRNA2 sequence; Cebpb-OE: mSSCs stably expressing the Cebpb full-length cDNA sequence. , , and .

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