Cebpb Regulates Skeletal Stem Cell Osteogenic Differentiation and Fracture Healing via the WNT/β-Catenin Pathway

Figure 4

Cebpb knockdown activated WNT signaling in mSSC osteogenic differentiation. (a) Expression of osteogenic differentiation signaling proteins determined by western blot in the Cebpb inhibition group and the control group. (b) Quantification of result (a). (c) Expression of WNT signaling and osteogenic differentiation proteins determined by western blot in the control, Cebpb inhibition, and WNT signaling inhibition groups. (d) Quantification of result (c). (e) Alizarin red S staining and alkaline phosphatase staining of mSSCs in the control, Cebpb inhibition, and WNT signaling inhibition groups. Scale bar: 200 μm. (f) Quantification of alizarin red S staining of result (e). (g) Quantification of alizarin red S staining of result (e). Cebpb-sh: mSSCs stably expressing the Cebpb-shRNA1 and Cebpb-shRNA2 sequence. compared with the control group, compared with the control group, compared with the control group, ^# compared with the Cebpb-sh group, ^## compared with the Cebpb-sh group, and ^### compared with the Cebpb-sh group. IWR1: inhibitor of WNT signaling.

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