Mesenchymal Stromal Cells Mediate Clinically Unpromising but Favourable Immune Responses in Kidney Transplant Patients

Table 1

Lymphocyte population sets, cytokines, and biomarkers characterized.

Lymphocyte populations
Parent populations	Subsets	Phenotype
1. Mononuclear cells	1. CD3 lymphocytes (T cells)	CD3^+
2. CD3^+ T cells	2. CD4 lymphocytes (helper T cells)	CD3^+CD4^+
	3. CD8 lymphocytes (cytotoxic T cells)	CD3^+CD8^+
	4. Double-negative T cells (DN T)	CD3^+CD4^-CD8^-
3. CD3^+ CD4^+ T cells/helper T cells	5. Naive T (TNAI) cells	CD3^+CD4^+CD45RA^+CD45RO^-CD62L^+
	6. Effector T (TEFF) cells	CD3^+CD4^+CD45RA^+CD45RO^-CD62L^-
	7. Memory T (TMEM) cells	CD3^+CD4^+CD45RA^-CD45RO^+
	8. Effector memory T (TMEM-EM) cells	CD3^+CD4^+CD45RA^-CD45RO^+CD62L^-
	9. Central memory T (TMEM-CM) cells	CD3^+CD4^+CD45RA^-CD45RO^+CD62L^+
	10. Regulatory T cells (TREGS)	CD3^+CD4^+CD25^hiFoxP3^+CD127^lo
4. CD3^+CD8^+ T cells/cytotoxic T cells	11. Naive T (TNAI) cells	CD3^+CD8^+CD45RA^+CD45RO^-CD62L^+
	12. Effector T (TEFF) cells	CD3^+CD8^+CD45RA^+CD45RO^-CD62L^-
	13. Memory T (TMEM) cells	CD3^+CD8^+CD45RA^-CD45RO^+
	14. Effector memory T (TMEM-EM) cells	CD3^+CD8^+CD45RA^-CD45RO^+ CD62L^-
	15. Central memory T (TMEM-CM) cells	CD3^+CD8^+CD45RA^-CD45RO^+ CD62L^+
5. CD19^+ B cells	16. Virgin naive (bm1) B cells	CD19^+IgD^+CD38^-
	17. Activated naive (bm2) B cells	CD19^+IgD^+CD38^+
	18. Pregerminal (bm2) B cells	CD19^+IgD^+CD38^hi
	19. Germinal centre (GC) (bm3+4) B cells	CD19^+IgD^-CD38^hi
	20. Early memory (early bm5) B cells	CD19^+IgD^-CD38^+
	21. Late memory (late bm5) B cells	CD19^+IgD^-CD38^-
	22. Double-negative B (DN B) cells	CD19^+IgD^-CD27^-
	23. Naive B cells	CD19^+IgD^+CD27^-
	24. Switched B cells	CD19^+IgD^-CD27^+
	25. Unswitched B cells	CD19^+IgD^+CD27^+
6. CD19^+ B regulatory cells (BREGS)	26. Regulatory B (Breg) cells	CD19^+CD5^+CD1d^hi
	27. Transitional B (B10) cells	CD19^+CD27^+CD24^hi
	28. Immature transitional B (BIM) cells	CD19^+CD24^hiCD38^hi
Cytokines analysed
1. Interleukin-2 (IL-2)
2. Interleukin-4 (IL-4)
3. Interleukin-6 (IL-6)
4. Interleukin-10 (IL-10)
5. Interleukin-17A (IL-17A)
6. Interferon-γ (IFN-γ)
7. Tumor necrosis factor-α (TNF-α)
8. Transforming growth factor-β1 (TGF-β1)
Biomarkers analysed
1. Serum creatinine (Scr)