Stage-Dependent Regulation of Dental Pulp Stem Cell Odontogenic Differentiation by Transforming Growth Factor-β1

Figure 2

Effects of TGF-β1 on cell behavior (a) CCK-8 assay of DPSCs proliferation after treatment with a range of concentrations of TGF-β1 (0-5 ng/mL) for 1, 3, 5, and 7 days. (b) Flow cytometry analysis showing changes in the cell cycle of DPSCs following treatment with a range of concentrations of TGF-β1 for 48 h. (c) Cell apoptosis following stimulation with the TGF-β1 concentrations for 48 h evaluated by flow cytometry. (SD), , statistically significant difference at the levels , , and (ANOVA) compared with the control group. CCK-8: cell counting kit-8; DPSCs: dental pulp stem cells; TGF-β1: transforming growth factor-β1.

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