Stage-Dependent Regulation of Dental Pulp Stem Cell Odontogenic Differentiation by Transforming Growth Factor-β1

Figure 4

Effect of TGF-β1 on odonto-/osteo-genic-related marker gene and protein expression in DPSCs in vitro (a) The expression levels of COL1A1, DSPP, OCN, BSP, and DMP-1 were detected by qRT-PCR at day 7. (b) Relative gene expression levels of the genes at day 14. (c) Western blot analysis and quantification of proteins expression at day 7 after TGF-β1 exposure. (d) Analysis of protein expression after 14 days. (SD), , statistically significant difference at the levels , , and (ANOVA) compared with the control group. BSP: bone sialoprotein; COL1A1: collagen type 1 alpha 1; DMP-1: dentin matrix protein 1; DPSCs: dental pulp stem cells; DSPP: dentin sialophosphoprotein; OCN: osteocalcin; TGF-β1: transforming growth factor-β1.

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