The Proangiogenic Potential of Rat Adipose-Derived Stromal Cells with and without Cell-Sheet Induction: A Comparative Study

Figure 3

Morphological and histological staining of ASC sheets after the induction of vitamin C. (a) The edge of the ASC sheets began to curl slightly on day 7 of cell sheet induction (red arrows indicated the curly margins of ASC sheets). (b) ASC sheets could be easily detached by tweezers. (c) Histological staining of ASC sheets on days 1, 4, 7, and 10 of cell sheet induction, respectively. By HE staining, the cell nuclei were dyed blue and the collagen fibers pale pink. By Sirius Red staining, the collagen fibers were dyed red, and by Masson staining, the collagen fibers were stained blue. Scale bars: 20 μm.

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