Delayed Supplementation Strategy of Extracellular Vesicles from Adipose-Derived Mesenchymal Stromal Cells with Improved Proregenerative Efficiency in a Fat Transplantation Model

Figure 1

Schematic representation of experimental design and its potential mechanism. (a) ADSCs were cultured and the supernatant was collected. ADSC-EV was obtained by ultracentrifugation technique. Subsequently, we subcutaneously injected human fat tissue into nude mice followed by ADSC-EV injection at 1 week after fat grafting. (b) The scheme shows that, at 1-week after grafting, endothelial cells derived from the recipient are recruited to form the new vessels in transplants and the inflammatory reaction subsides, making the environment more conducive for ADSC-EV to display its regenerative effects.