Research Article

Delayed Supplementation Strategy of Extracellular Vesicles from Adipose-Derived Mesenchymal Stromal Cells with Improved Proregenerative Efficiency in a Fat Transplantation Model

Figure 2

Identification and internalization of ADSC-EVs. (a) Transmission electron microscopy image of ADSC-EV after negative staining shows EVs are on average about 200 nm. . (b) Picture of ADSC-EVs labeled with the red fluorescent dye PKH26. . (c) Nanosight profile of ADSC-EVs. Their sizes and concentrations are shown. Diameter distribution peaks at around 166 nm. (d) Western blot results showing positive expression of TSG101 (EV surface markers) and (e) VEGF in ADSC-EVs. (f) PKH26-labelled ADSC-EVs (red fluorescence) were added to HUVECs (nuclei were stained by DAPI); representative structured illumination microscopy images are shown. .
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