Constructing Tissue-Engineered Dressing Membranes with Adipose-Derived Stem Cells and Acellular Dermal Matrix for Diabetic Wound Healing: A Comparative Study of Hypoxia- or Normoxia-Culture Modes

Figure 4

(a) Live/dead cell analysis for the ADM membrane on which ADSCs had been seeded and cultured under normoxic or hypoxic condition. Representative images show the live (green) and dead (red) ADSCs in the TCPS and the ADM membrane cultured under normoxic or hypoxic condition and the viability analysis for the cells on the TCPS and ADM membrane, per group. (b) Scanning electron micrographs of the ADM membrane on which ADSCs had been cocultured under normoxic or hypoxic condition for 48 hours. (c) Proliferation of HUVECs influenced by H medium and N medium was evaluated with Cell Counting Kit-8 (CCK8) assay at 0,1, 2, and 3 days after cell seeding, per group. (d) Representative images of scratch on HUVECs at 0, 12, and 24 hours after treated by H medium and N medium. The migration of HUVECs was significantly increased by H medium, per group. (e) In vitro tube formation of HUVECs at 12 hours under the stimulation of C medium and H medium.  μm, per group. (f) The content of angiogenic-related growth factors (VEGF-A, HGF, and FGF) in culture medium from hypoxia- or normoxia-preconditioned ADSCs determined by ELISA, the FGF content was too low to be measured. per group. All data are shown as (, , and ).

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