Research Article

Autophagy Regulates Osteogenic Differentiation of Human Periodontal Ligament Stem Cells Induced by Orthodontic Tension

Figure 2

Changes in osteogenic and autophagy levels under mechanical stretch. (a) Cell proliferation activity detected using the CCK-8 kit. (b–f) LC3-, ATG7-, ALP-, RUNX2-, and OPN-relative mRNA expression levels assessed by real-time PCR at 0, 1, 3, 6, 12, and 24 h. . OD: optical density; PCR: polymerase chain reaction.
(a)
(b)
(c)
(d)
(e)
(f)