Autophagy Regulates Osteogenic Differentiation of Human Periodontal Ligament Stem Cells Induced by Orthodontic Tension

Figure 3

Changes in osteogenic and autophagy levels under mechanical stretch. (a–c) LC3- and ATG7-relative protein expression levels assessed by western blotting at 0, 1, 3, 6, 12, and 24 h. (d–f) RUNX2- and OPN-relative protein expression levels assessed by western blotting at 0, 1, 3, 6, 12, and 24 h. (g, h) Autophagy activation was monitored by transmission electron microscopy, indicated by red arrows. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)