Small Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhanced Proangiogenic Potential of Cardiac Fibroblasts via Angiopoietin-Like 4

Figure 2

The sEVs enhanced proangiogenic potential of CFs. Primary CFs were treated with LPS (100 ng/mL) and LPS+sEVs (200 μg/mL) for 24 h, and supernatants were collected. HUVECs were treated with the supernatants derived from pretreated CFs. (a) Proliferation of HUVECs was detected by the CCK-8 assay. (b) The mRNA expression of cyclin D1 was measured by qRT-PCR. (c) Cyclin D1 protein expression was detected by western blotting. (d, f) Migration of HUVECs in different treatment groups was tested by the wound healing assay (d) and Transwell migration assay (f). Scale bar: 500 μm (d) and 100 μm (f). (e) Quantitative analysis of 12 h migration rate in (d). (g) Quantitative analysis of migrated cells in (f). (h–j) Tube formation ability of HUVECs was measured by the tube formation assay (h). Quantitative analysis of the total branching points (i) and tube formation numbers (j). Scale bar: 500 μm. , , , and .

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