Small Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhanced Proangiogenic Potential of Cardiac Fibroblasts via Angiopoietin-Like 4

Figure 3

Angptl4 was increased in CFs treated with sEVs. Primary CFs were treated with LPS (100 ng/mL) and LPS+sEVs (200 μg/mL) for 24 h. mRNA expression of these two groups was compared via RNA-seq analysis. (a) Volcano map of DEGs. (b) Relative fold change of the top 20 angiogenesis-related genes for enrichment analysis. (c) Angptl4 mRNA expression was viewed by qRT-PCR. (d, e) Protein expression of Angptl4 was detected by western blotting. (f, g) Immunofluorescence was used to observe Angptl4 expression. Scale bar: 100 μm. , , , and .

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