Small Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhanced Proangiogenic Potential of Cardiac Fibroblasts via Angiopoietin-Like 4

Figure 4

Inhibition of Angptl4 in primary CFs and sEVs resulted in decreased angiogenesis. (a) Angptl4 protein expression in hucMSCs and sEVs was detected by western blotting. (b, c) hucMSCs (b) and CFs (c) were transfected with Angptl4 siRNA, respectively, and expression of Angptl4 was measured by western blotting. (d) Angptl4 and CD81 expression in sEVs was observed by western blotting after transfection with siRNA. (e) Angptl4 expression in CFs was detected by western blotting after Angptl4 in CFs and in sEVs was knocked down with siRNA. (f) HUVECs were treated with the supernatants derived from pretreated CFs. The proliferation of HUVECs was measured by the CCK-8 assay. (g, h) The migration of HUVECs was detected by the Transwell migration assay (g). Scale bar: 100 μm. Quantitative analysis of the migrated cells (h). (i–k) Tube formation ability of HUVECs was measured by tube formation assay (i). Quantitative analysis of the total branching points (j) and tube formation numbers (k). Scale bar: 500 μm. and . ns: no significant difference between two groups.

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