Small Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhanced Proangiogenic Potential of Cardiac Fibroblasts via Angiopoietin-Like 4

Figure 5

Proangiogenic potential of CFs enhanced by sEVs was associated with β-catenin. (a) β-Catenin expression in CFs was detected by western blotting after Angptl4 in CFs and in hucMSCs was knocked down with siRNA. (b) After using the β-catenin inhibitor ICG001 (0, 5, 10, and 15 μM), primary CFs were treated with LPS (100 ng/mL) and LPS+sEVs (200 μg/mL) for 24 h and supernatants were collected to culture HUVECs. The proliferation of HUVECs was measured by the CCK-8 assay. (c) The migration of HUVECs was measured by the Transwell migration assay. Scale bar: 100 μm. (d) Quantitative analysis of migrated cells. (e–g) Tube formation ability of HUVECs was evaluated by the tube formation assay (e). Scale bar: 500 μm. Quantitative analysis of the total branching points (f) and tube formation numbers (g). , , , and .

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