Golgin Subfamily A Member 5 Is Essential for Production of Extracellular Matrix Proteins during TGF-β1-Induced Periodontal Ligament-Fibroblastic Differentiation

Figure 3

GOLGA5/LG11 is a PDL-fibroblast specific Golgi marker. (a) GOLGA5/LG11 accumulated in PDL region of tooth root. Immunohistochemistry analysis using human (A) and fetal mouse (B) tooth slices. Anti-PLAP-1 antibody was used as the positive marker of periodontal ligament. (b) GOLGA5/LG11 was detected in membrane fraction of TGF-β1-induced PDL-fibroblastic cells. Total: total cell extract; cyto: cytosolic phase; mem: membrane fraction. (c) Subcellular localization of endogenous GOLGA5/LG11 in undifferentiated hPDLSCs. The representative organelle markers, such as GM130, calnexin, integrin β5, and vinculin, were detected by Cy3 for red colors (Cy3-markers), whereas GOLGA5/LG11 was detected by FITC for green colors. Nuclei were detected by DAPI staining. (d) Subcellular localization of endogenous GOLGA5/LG11 and GM130 during TGF-β1-induced fibroblastic differentiation. hPDLSCs grown on coverslips were treated with TGF-β1 for the indicated times (4 and 9 days) and applied for immunocytochemistry. As the differentiation progressed, the more cells in which LG11 and GM130 were separated from each other were detected (arrowheads). Areas marked with asterisks ( and ) in (A) are enlarged and shown in (B).

(a)

(b)

(c)

(d)