Golgin Subfamily A Member 5 Is Essential for Production of Extracellular Matrix Proteins during TGF-<i>β</i>1-Induced Periodontal Ligament-Fibroblastic Differentiation

<div>GOLGA5/LG11 is involved in synthesis of ECM proteins. (a) Inhibition of TGF-<i>β</i>1-induced fibroblastic differentiation by SB431542 treatment caused Golgi fragmentation. The structures of Golgi apparatus detected by Cy3-labeled GM130. Golgi marked with arrowheads in (A) are enlarged and shown in (B). (b) Synthesis of ECM proteins such as collagen type 1 and fibronectin in PDL-fibroblastic progenitors was significantly blocked by decrease in GOLGA5 amount, as well as GOLGA5/LG11, collagen type 1<i>α</i>1, collagen type 1<i>α</i>2, and fibronectin in fibroblastic cells were completely decreased by GOLGA5 depletion by siRNA (A) and SB431542 treatment (B). (C) Immunocytochemistry of endogenous collagen type 1. Expression of collagen type 1 in cells was detected by Cy3 signals. Nuclei were detected by DAPI staining.</div>

Stem Cells International

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Figure 5

Figure 5: Golgin Subfamily A Member 5 Is Essential for Production of Extracellular Matrix Proteins during TGF-<i>β</i>1-Induced Periodontal Ligament-Fibroblastic Differentiation 

Figure 5 | Golgin Subfamily A Member 5 Is Essential for Production of Extracellular Matrix Proteins during TGF-β1-Induced Periodontal Ligament-Fibroblastic Differentiation 