Energy Metabolism and Lipidome Are Highly Regulated during Osteogenic Differentiation of Dental Follicle Cells

Figure 3

Expression of lipid metabolism markers during osteogenic differentiation of DFCs. DFCs were cultured in osteogenic differentiation medium (ODM), BMP2 differentiation medium, or control medium (DMEM) for one, seven, 14, and 28 days before protein expression of acetyl-CoA carboxylase, phospho-acetyl-CoA carboxylase, ATP-citrate lyase, phospho-ATP-citrate lyase, acetyl-CoA synthetase, acyl-CoA synthetase, fatty acid synthase, and elongation of very long chain fatty acids protein 6 (ELOVL6) was determined by western blot analysis. Quantification results and statistical analysis are shown in the Supplementary Table 2.