Energy Metabolism and Lipidome Are Highly Regulated during Osteogenic Differentiation of Dental Follicle Cells

Figure 4

Regulation of membrane lipids during osteogenic differentiation of DFCs. DFCs were cultured in osteogenic differentiation medium (ODM), BMP2 differentiation medium, or control medium (DMEM) for one, seven, 14 and 28 days before the lipidome was analyzed by MS. This figure shows the regulation of total lipids (a) and the fraction of phosphatidylethanolamines (PE, b), phosphatidylcholines (PC, c), phosphatidylinositols (PI, d), phosphatidylserines (PS, e), phosphatidylethanolamine-based plasmalogens (PE P, f), phosphatidylcholine-ethers (PC O, g), sphingomyelins (SM, h), and free cholesterol (FC, i). Data points show , and Student’s -test was performed to compare differentiation medium with control medium at the same time point. , , . Asterisks relate to the differentiation medium with data points and line in same color.

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