Energy Metabolism and Lipidome Are Highly Regulated during Osteogenic Differentiation of Dental Follicle Cells

Figure 6

Regulation of storage lipids and diacylglycerol during osteogenic differentiation of DFCs. DFCs were cultured in osteogenic differentiation medium (ODM), BMP2 differentiation medium, or control medium (DMEM) for one, seven, 14, and 28 days before the lipidome was analyzed by MS. (a–c) The line charts show the fraction of diacylglycerols (DG, a), triacylglycerols (TG, b), and cholesteryl esters (CE, c). Data points show , and Student’s -test was performed to compare differentiation medium with control medium at the same time point. , , . Asterisks relate to the differentiation medium with data points and line in same color. (d, e) Furthermore, the composition of DG species (d) and TG species (e) in cells treated with ODM at the different time points is shown. Only species with an abundance of more than 2% for at least one time point are shown as , and one-way ANOVA was performed to determine significant differences in the fraction of a specific species at different time points. , .

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