Energy Metabolism and Lipidome Are Highly Regulated during Osteogenic Differentiation of Dental Follicle Cells

Figure 7

Regulation of osteogenic differentiation in DFCs after inhibition of fatty acid synthesis. DFCs were cultured in osteogenic differentiation medium (ODM), BMP2 differentiation medium, or control medium (DMEM) and simultaneously treated with 5 μg/ml fatty acid synthesis inhibitors TOFA or C75 or vehicle control for different time periods before evaluation of osteogenic differentiation markers: (a, b) Gene expressions of COL1A2 (a) and RUNX2 (b) relative to gene expression of GAPDH were determined after three days treatment by RT-qPCR. (c) Activity of alkaline phosphatase (ALP) relative to total protein was measured after seven days treatment. (d) Mineralization of extracellular matrix was determined by Alizarin Red staining after 28 days treatment. Photographic pictures of the staining are shown below the bar chart which shows the relative quantification results as . Student’s -tests were performed to compare inhibitor treatment with the vehicle control. , , .

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