FAIM Enhances the Efficacy of Mesenchymal Stem Cell Transplantation by Inhibiting JNK-Induced c-FLIP Ubiquitination and Degradation

Figure 4

c-FLIP is required for the antiapoptotic effect of FAIM. MSCs_Vec and MSCs_FLIP were transfected with si-FAIM and exposed to OGD. (a–b) Annexin V-APC/PI staining was performed, and flow cytometry was used to determine the apoptosis rate. (c–d) TUNEL staining of MSCs_Vec and MSCs_FLIP after FAIM knockdown followed by OGD treatment for 12 h. (e–f) Cleaved caspase 3 protein expression was detected by Western blotting. (g–h) Annexin V-APC/PI staining of MSCs_FAIM transfected with si-FLIP or si-Scr under OGD conditions. (i–j) TUNEL staining of MSCs_FAIM after FLIP knockdown or control treatment followed by OGD treatment for 12 h. (k–l) Cleaved caspase 3 protein expression was measured by Western blotting. The data are shown as the . indicates not significant; denotes .

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