Human Mesenchymal Stem Cells Derived from the Placenta and Chorion Suppress the Proliferation while Enhancing the Migration of Human Breast Cancer Cells

Figure 5

The effect of soluble factors on the migration of human breast cancer MDA-MB231 cells. (a) Representative micrographs of migratory MDA-MB231 cells induced by 10%, 25%, 50%, and 75% conditioned medium derived from CH-hMSCs (CH-CM) PL-hMSCs (PL-CM) determined by the transwell migration assay. CH-CMs were prepared from 3 CH-hMSCs (CH9, CH15, and CH16), and PL-CMs were prepared from 3 PL-hMSCs (PL11, PL14, and PL17). MDA-MB231 cells induced by DMEM medium +30% FBS served as controls. (b) Graphs show the number of migratory MDA-MB231 cells induced by 10%, 25%, 50%, and 75% conditioned media derived from CH-CM and PL-CM determined by transwell migration assay. Data are presented as of three experiments. CH-CMs were prepared from 3 CH-hMSCs (CH9, CH15, and CH16), and PL-CMs were prepared from 3 PL-hMSCs (PL11, PL14, and PL17). MDA-MB231 cells induced by DMEM medium +30% FBS served as controls. vs. control, vs. control. (c) Graphs show the number of migratory MDA-MB231 cells induced by 10%, 25%, 50%, and 75% conditioned media derived from CH-hMSC-CM (average of CH9, CH15, and CH16) and PL-hMSC-CM (average of PL11, PL14, and PL17). Data are presented as of three hMSC-CMs. MDA-MB231 induced by DMEM medium +30% FBS served as controls. vs. control, vs. control.

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