Enhanced Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood and Using Their Mesoderm Differentiation Ability to Regenerate Infarcted Myocardium

Figure 4

Comparison of histone tail modifications according to the three germline differentiation stages of each iPSC. (a) Histone tail modification levels of the transcription site of brachyury T during mesodermal differentiation (day 2 (D2)) of the three different cell types were compared using a chromatin immunoprecipitation (ChIP) assay. The H3K4me3, H3K9ac, and H3K27me3 levels of brachyury T were analyzed (, , and ; ss: statistically significant; ns: statistically not significant). (b) Histone tail modification levels of the GSC transcription site during endodermal differentiation (day 2 (D2)) of the three different cell types were compared by ChIP assay. H3K4me3, H3K9ac, and H3K27me3 of GSC were analyzed. (c) Histone tail modification levels of the Sox1 transcription site during ectodermal differentiation (day 2 (D2)) of the three different cell types were compared by ChIP assay. H3K4me3, H3K9ac, and H3K27me3 of Sox1 were analyzed. (d) After treatment with the H3K4 KMT inhibitor (50 μM MM102; D1i, D2i) during mesodermal differentiation (day 1 (D1), day 2 (D2)), H3K4me3 level of the brachyury T transcription site was compared in each cell type. (e) After treatment with the H3K9 KMT inhibitor (100 nM chaetocin; D1i, D2i) during mesodermal differentiation (day 1 (D1), day 2 (D2)), the H3K9ac level of brachyury T transcription site was compared in each cell type. (f) Brachyury T gene expression following H3K4 KTM inhibitor (MM102) treatment on mesoderm differentiation day 1 and day 2 was measured in each cell type using real-time PCR. (g) Brachyury T gene expression following H3K4 KTM inhibitor (chaetocin) treatment on mesoderm differentiation day 1 and day 2 was measured in each cell type using real-time PCR.

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