Dopamine Suppresses Osteogenic Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells via AKT/GSK-3β/β-Catenin Signaling Pathway

Figure 2

DA does not influence the apoptosis and proliferation of rBMSCs. (a) rBMSCs were treated with different concentrations of DA for 2 days. Flow cytometric analysis of apoptosis using Annexin V-FITC/PI staining. (b) Apoptosis rate analysis. (c) Effect of DA on the proliferation of rBMSCs for 1, 3, 5, and 7 days evaluated by CCK-8 assay. (d) Effect of DA on the proliferation of rBMSCs for 1 day evaluated by EdU assay. Proliferating nuclei were labeled with EdU (green), and the nuclei of all cells were labeled with Hoechst (blue). (e) The cell proliferation ratio of the indicated groups was expressed as the ratio of EdU-positive cells to total Hoechst-positive cells. Scale bar, 100 μm. ns: no significance.

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